Salinas Research Group
Dr. Gabriela Salinas
Group Leader
gsalina@gwdg.de
Roya Abbaszadeh
PhD student
roya.abbaszadeh@stud.uni-goettingen.de
Dr. Emre Taylan Duman
Bioinformatics Scientist
emretaylan.duman@med.uni-goettingen.de
Jacqueline Fink
Technician
jacqueline.fink@med.uni-goettingen.de
Fabian Ludewig
Technician
fabian.ludewig@med.uni-goettingen.de
Susanne Luthin
Technician
sluthin@gwdg.de
Dr. Maren Sitte
Bioinformatics Scientist
maren.sitte@med.uni-goettingen.de
Focus
Next-generation sequencing (NGS) can now be considered an established analysis technology for research applications across the life sciences and especially in clinical environments, where it facilitates diagnostics enabling personalized therapeutic approaches, but is also accompanied by new technological, legal, and ethical challenges. The aim of our group is to facilitate the latest and state-of-the-art NGS technologies, methods, platforms and to provide substantial bioinformatic expertise to our cooperation partners and users to achieve reliable results.
We also use the results and advances made in our research projects to steadily improve and expand the resources and services that we offer at NIG, NGS Integrative Genomics Core Unit, at the University Medical Center Göttingen.
Projects
Development of a unique platform for scRNA-Seq by a combination of CellenONE X1 and ICELL8
NGS-based technologies for genomics, transcriptomics, and epigenomics are now increasingly focused on the characterization of individual cells. Single-cell sequencing (SCS) allows to uncover complex heterogeneous cell populations and regulatory relationships between cell-types and to track the trajectory of distinct cell sub-types in a tissue. All of these questions are central to several projects of our CRC1002, which aims at characterizing the impact of a disease condition, different treatments and/or target modulation on different cell populations of the heart in human diseases and animal models. Our group is working on the development of platforms for single-cell NGS to improve the first and crucial step, the single-cell isolation. Dispensing the cells by using the CellenONE X1 instrument allow us to capture cells with a high efficiency (nearly 100%), high cell viability, high precision, high-throughput mode and well documentation and visualization of the isolated cells.
To guarantee a high-throughput approach, individual cells are collected from the CellenONE X1 to one 5184-well plate from Takara, the ICELL8 plate with or without label indexing depending on the library preparation required. Three different protocols for scRNA-Seq can be conducted: a) a Drop-Seq based approach, b) a full-length mRNA Seq approach, and c) a total RNA Seq approach by using the SMART-Technology of Takara. The system allows the performance of DNA sequencing methods as well as Nextera-based libraries (ATAC-Seq).
Direct RNA Seq using the Oxford Nanopore technology
The MinION sequencer is a portable long-read sequencer. After several rounds of updates, its sequence accuracy has now exceeded 90% and the expected number of reads per flow cell is over hundreds of thousands per flow cell. The read length has also been improved, achieving a N50 read length of more than 100 kb. We currently test the application of MinION to RNA-Seq using R7.3 MinION flow cell and single-cell RNA-Seq using R7.3 and 9.4 flow cells.
In situ RNA sequencing with preservation of spatial information
This technique is an innovative approach that can provide single-cell transcriptomic or highly multiplexed gene expression information for the analysis of tissues or clinical samples (biopsy) without need of tissue dissociation protocols. Specifically for clinical research studies this technique will be transformative.
Protocols being established
Currently the following protocols are being established:
- Nuclei isolation for single-nucleus RNA-Seq (SNS) for the ICELL8
- Nuclei isolation for single-nucleus RNA-Seq (SNS) for the 10 x Genomics
- Single-cell total RNA-Seq approaches
Deep learning techniques for association studies of transcriptome and systems dynamics in tissue morphogenesis
This initiative brings together scientists from the disciplines of information theory, theoretical neuroscience, transcriptomics, and cell and developmenal biology to combine for the first time imaging and expression data for a better understanding of the relations that exist between gene expression in single cells and the behavior of cell aggregates. It aims at the automation of dynamic tissue reconstruction from large-scale live imaging data by means of deep learning for real-time identification of single cells at key sites of the embryo and their transcriptomic analysis.
The project is funded by the Ministry of Science and Culture of Lower Saxony (MWK) and Volkswagen Foundation under the call “Big Data in the Life Sciences of the Future”.
CRU 5002 “Deciphering genome dynamics for subtype-specific therapy in pancreatic cancer”
Central Project CP2
In the framework of the DFG-funded Clinical Research Unit CRU 5002 the Biomedical Informatics Support Platform (BISP) of CP2 is responsible for providing IT structures, bioinformatic methods and pipelines to store, analyse and integrate the numerous phenotypic, genomic and functional data generated in SP1-7 and CP1. Further, the data of the CRU is merged and analyzed in the context of the comprehensive clinical annotations gathered in theMolPAC program. In this core project Next-Generation-Sequencing studies and transcriptome analyses are performed on a central platform for all genomic analyses required throughout the consortium as well as for translational PDAC models established in CP1. In addition, CP2 offers statistical consulting to all scientific sub projects for the meaningful planning and execution of their genomic experiments. CP2 is responsible for the execution of comprehensive bioinformatic analyses and the qualified interpretation of genomic data. Finally, CP2 integrates molecular subtyping data collected within the CRU 5002 with published data on PDAC subgroups and supports CP1 in comparing PDAC PDX-models and organoids for their subtype consistency.
CRC 1002 “Modulatory Units in Heart Failure”
The DFG-funded collaborative research center (CRC) 1002 strives to gain a deeper understanding of the pathophysiology of heart failure and aims at identifying new and more effective options for its treatment. The researchers investigate selected signalling pathways that organize communication between the various cells in the heart and the smallest functional entities in heart cells, called functional mcrodomains, and play an important role in the development of heart failure.
Publications Salinas Research Group
2023
N4-hydroxycytidine, the active compound of Molnupiravir, promotes SARS-CoV-2 mutagenesis and escape from a neutralizing nanobody
Zibat A, Zhang X, Dickmanns A, Stegmann KM, Dobbelstein AW, Alachram H, Soliwoda R, Salinas G, Groß U, Görlich D, Kschischo M, Wollnik B, Dobbelstein M
iScience 2023 Aug 30;26(10):107786. doi: 10.1016/j.isci.2023.107786.
Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis
Hirschhäuser A, Molitor D, Salinas G, Großhans J, Rust K, Bogdan S
Development 2023 Sep 7:dev.201767. doi: 10.1242/dev.201767. Epub ahead of print.
Elastin stabilization prevents impaired biomechanics in human pulmonary arteries and pulmonary hypertension in rats with left heart disease
Kucherenko MM, Sang P, Yao J, Gransar T, Dhital S, Grune J, Simmons S, Michalick L, Wulsten D, Thiele M, Shomroni O, Hennig F, Yeter R, Solowjowa N, Salinas G, Duda GN, Falk V, Vyavahare NR, Kuebler WM, Knosalla C
Nat Commun 2023 Jul 21;14(1):4416. doi: 10.1038/s41467-023-39934-z.
Surveillance of 3′ mRNA cleavage during transcription termination requires CF IB/Hrp1
Li J, Querl L, Coban I, Salinas G, Krebber H
Nucleic Acids Res 2023 Sep 8;51(16):8758-8773. doi: 10.1093/nar/gkad530.
Concurrent Activation of Both Survival-Promoting and Death-Inducing Signaling by Chloroquine in Glioblastoma Stem Cells: Implications for Potential Risks and Benefits of Using Chloroquine as Radiosensitizer
Müller A, Weyerhäuser P, Berte N, Jonin F, Lyubarskyy B, Sprang B, Kantelhardt SR, Salinas G, Opitz L, Schulz-Schaeffer W, Giese A, Kim EL
Cells 2023 Apr 30;12(9):1290. doi: 10.3390/cells12091290.
Blood T Helper Memory Cells: A Tool for Studying Skin Inflammation in HS?
Witte K, Schneider-Burrus S, Salinas G, Mössner R, Ghoreschi K, Wolk K, Sabat R
Int J Mol Sci 2023 May 16;24(10):8854. doi: 10.3390/ijms24108854.
Comprehensive identification of gene expression fingerprints and biomarkers of sexual endocrine disruption in zebrafish embryo
Ayobahan SU, Alvincz J, Reinwald H, Strompen J, Salinas G, Schäfers C, Eilebrecht E, Eilebrecht S
Ecotoxicol Environ Saf 2023 Jan 15;250:114514. doi: 10.1016/j.ecoenv.2023.114514. Epub 2023 Jan 4.
Combining ferroptosis induction with MDSC blockade renders primary tumours and metastases in liver sensitive to immune checkpoint blockade
Conche C, Finkelmeier F, Pešić M, Nicolas AM, Böttger TW, Kennel KB, Denk D, Ceteci F, Mohs K, Engel E, Canli Ö, Dabiri Y, Peiffer KH, Zeuzem S, Salinas G, Longerich T, Yang H, Greten FR
Gut 2023 Jan 27:gutjnl-2022-327909. doi: 10.1136/gutjnl-2022-327909. Online ahead of print.
Prognostic factor identification by screening changes in differentially expressed genes in oral squamous cell carcinoma
Schminke B, Shomroni O, Salinas G, Bremmer F, Kauffmann P, Schliephake H, Oyelami F, Rahat MA, Brockmeyer P
Oral Dis 2023 Jan;29(1):116-127. doi: 10.1111/odi.13879. Epub 2021 May 5.
Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling
Schoger E, Bleckwedel F, Germena G, Rocha C, Tucholla P, Sobitov I, Möbius W, Sitte M, Lenz C, Samak M, Hinkel R, Varga ZV, Giricz Z, Salinas G, Gross JC, Zelarayán LC
Commun Biol 2023 Jan 21;6(1):79. doi: 10.1038/s42003-022-04402-9.
2022
Heat stress leads to rapid lipid remodeling and transcriptional adaptations in Nicotiana tabacum pollen tubes
Krawczyk HE, Rotsch AH, Herrfurth C, Scholz P, Shomroni O, Salinas-Riester G, Feussner I, Ischebeck T
Plant Physiol 2022 Jun 1;189(2):490-515. doi: 10.1093/plphys/kiac127.
Neutrophilic granulocyte-derived B-cell activating factor supports B cells in skin lesions in hidradenitis suppurativa
Sabat R, Šimaitė D, Gudjonsson JE, Brembach TC, Witte K, Krause T, Kokolakis G, Bartnik E, Nikolaou C, Rill N, Coulibaly B, Levin C, Herrmann M, Salinas G, Leeuw T, Volk HD, Ghoreschi K, Wolk K
J Allergy Clin Immunol 2022 Dec 5:S0091-6749(22)01621-9. doi: 10.1016/j.jaci.2022.10.034. Online ahead of print.
Engineered skeletal muscle recapitulates human muscle development, regeneration and dystrophy
Shahriyari M, Islam MR, Sakib SM, Rinn M, Rika A, Krüger D, Kaurani L, Gisa V, Winterhoff M, Anandakumar H, Shomroni O, Schmidt M, Salinas G, Unger A, Linke WA, Zschüntzsch J, Schmidt J, Bassel-Duby R, Olson EN, Fischer A, Zimmermann WH, Tiburcy M
J Cachexia Sarcopenia Muscle 2022 Dec;13(6):3106-3121. doi: 10.1002/jcsm.13094. Epub 2022 Oct 18.
Short-Term Test for Toxicogenomic Analysis of Ecotoxic Modes of Action in Lemna minor
Loll A, Reinwald H, Ayobahan SU, Göckener B, Salinas G, Schäfers C, Schlich K, Hamscher G, Eilebrecht S
Environ Sci Technol 2022 Aug 16;56(16):11504-11515. doi: 10.1021/acs.est.2c01777. Epub 2022 Aug 4.
Prdm12 modulates pain-related behavior by remodeling gene expression in mature nociceptors
Latragna A, Sabaté San José A, Tsimpos P, Vermeiren S, Gualdani R, Chakrabarti S, Callejo G, Desiderio S, Shomroni O, Sitte M, Kricha S, Luypaert M, Vanhollebeke B, Laumet G, Salinas G, Smith ESJ, Ris L, Bellefroid EJ
Pain 2022 Aug 1;163(8):e927-e941. doi: 10.1097/j.pain.0000000000002536. Epub 2021 Dec 24.
HNF1B Alters an Evolutionarily Conserved Nephrogenic Program of Target Genes
Grand K, Stoltz M, Rizzo L, Röck R, Kaminski M, Salinas G, Getwan M, Naert T, Pichler R, Lienkamp S
J Am Soc Nephrol. 2022 Dec 15:ASN.2022010076. doi: 10.1681/ASN.2022010076. Epub ahead of print.
Detailed phenotypic and functional characterization of CMV-associated adaptive NK cells in rhesus macaques
Hasan MZ, Höltermann C, Petersen B, Schrod A, Mätz-Rensing K, Kaul A, Salinas G, Dressel R, Walter L
Front Immunol. 2022 Nov 25;13:1028788. doi: 10.3389/fimmu.2022.1028788.
Small and long RNA transcriptome of whole human cerebrospinal fluid and serum as compared to their extracellular vesicle fractions reveal profound differences in expression patterns and impacts on biological processes
Michel U, Shomroni O, Müller B, Lange P, Salinas G, Bähr M, Koch JC
J Transl Med 2022 Sep 8;20(1):413. doi: 10.1186/s12967-022-03612-3.
Gene-expression profiles of pretreatment biopsies predict complete response of rectal cancer patients to preoperative chemoradiotherapy
Emons G, Auslander N, Jo P, Kitz J, Azizian A, Hu Y, Hess CF, Roedel C, Sax U, Salinas G, Stroebel P, Kramer F, Beissbarth T, Grade M, Ghadimi M, Ruppin E, Ried T, Gaedcke J
Br J Cancer 2022 May 21. doi: 10.1038/s41416-022-01842-2. Online ahead of print.
Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming during thermal adaptation
Hadadi N, Spiljar M, Steinbach K, Çolakoğlu M, Chevalier C, Salinas G, Merkler D, Trajkovski M.
Elife. 2022 May 17;11:e78556. doi: 10.7554/eLife.78556.
Calcium bursts allow rapid reorganization of EFhD2/Swip-1 cross-linked actin networks in epithelial wound closure
Lehne F, Pokrant T, Parbin S, Salinas G, Großhans J, Rust K, Faix J, Bogdan S.
Nat Commun 2022 May 6;13(1):2492. doi: 10.1038/s41467-022-30167-0.
IGF-1 Gene Transfer Modifies Inflammatory Environment and Gene Expression in the Caudate-Putamen of Aged Female Rat Brain
Falomir-Lockhart E, Dolcetti FJC, Herrera ML, Pennini J, Zappa Villar MF, Salinas G, Portiansky E, Spittau B, Lacunza E, Hereñú CB, Bellini MJ.
Mol Neurobiol. 2022 Jun;59(6):3337-3352. doi: 10.1007/s12035-022-02791-w. Epub 2022 Mar 19.
Toxicogenomic profiling after sublethal exposure to nerve- and muscle-targeting insecticides reveals cardiac and neuronal developmental effects in zebrafish embryos
Reinwald H, Alvincz J, Salinas G, Schäfers C, Hollert H, Eilebrecht S.
Chemosphere 2022 Mar;291(Pt 1):132746. doi: 10.1016/j.chemosphere.2021.132746.
Regenerative potential of epicardium-derived extracellular vesicles mediated by conserved miRNA transfer
Del Campo CV, Liaw NY, Gunadasa-Rohling M, Matthaei M, Braga L, Kennedy T, Salinas G, Voigt N, Giacca M, Zimmermann WH, Riley PR.
Cardiovasc Res. 2022 Jan 29;118(2):597-611. doi: 10.1093/cvr/cvab054.
A novel single-cell RNA-sequencing approach and its applicability connecting genotype to phenotype in ageing disease
Shomroni O, Sitte M, Schmidt J, Parbin S, Ludewig F, Yigit G, Zelarayan LC, Streckfuss-Bömeke K, Wollnik B, Salinas G
Sci Rep. 2022 Mar 8;12(1):4091. doi: 10.1038/s41598-022-07874-1.
Transcriptomic profiling of clobetasol propionate-induced immunosuppression in challenged zebrafish embryos
Essfeld F, Reinwald H, Salinas G, Schäfers C, Eilebrecht E, Eilebrecht S
Ecotoxicol Environ Saf 2022 Mar 15;233:113346. doi: 10.1016/j.ecoenv.2022.113346. Epub 2022 Feb 25.
The HIFα-Stabilizing Drug Roxadustat Increases the Number of Renal Epo-Producing Sca-1+ Cells
Jatho A, Zieseniss A, Brechtel-Curth K, Guo J, Böker KO, Salinas G, Wenger RH, Katschinski DM
Cells. 2022 Feb 21;11(4):753. doi: 10.3390/cells11040753.
2021
Transcriptome Analysis of Hypoxic Lymphatic Endothelial Cells Indicates Their Potential to Contribute to Extracellular Matrix Rearrangement
Becker J, Schwoch S, Zelent C, Sitte M, Salinas G, Wilting J
Cells. 2021 Apr 24;10(5).
Protein Signatures of NK Cell-Mediated Melanoma Killing Predict Response to Immunotherapies
Cappello S, Sung HM, Ickes C, Gibhardt CS, Vultur A, Bhat H, Hu Z, Brafford P, Denger A, Stejerean-Todoran I, Köhn RM, Lorenz V, Künzel N, Salinas G, Stanisz H, Legler T, Rehling P, Schön MP, Lang KS, Helms V, Herlyn M, Hoth M, Kummerow C, Bogeski I
Cancer Res 2021 Nov 1;81(21):5540-5554. doi: 10.1158/0008-5472.CAN-21-0164. Epub 2021 Sep 13.
Investigations on the Degradation of the Bile Salt Cholate via the 9,10-Seco-Pathway Reveals the Formation of a Novel Recalcitrant Steroid Compound by a Side Reaction in Sphingobium sp. Strain Chol11
Feller FM, Eilebrecht S, Nedielkov R, Yücel O, Alvincz J, Salinas G, Ludwig KC, Möller H, Philipp B
Microorganisms 2021 Oct 14;9(10):2146. doi: 10.3390/microorganisms9102146.
Screening for potential targets to reduce stenosis in bioprosthetic heart valves
Foth R, Shomroni O, Sigler M, Hörer J, Cleuziou J, Paul T, Eildermann K
Sci Rep. 2021 Jan 28;11(1):2464. doi: 10.1038/s41598-021-81340-2.
Restructuring of the male mice peripheral circadian network after bariatric surgery
Neumann AM, Geißler C, Pilorz V, Olejniczak I, Lewis AG, Seeley RJ, Shomroni O, Salinas-Riester G, Kirchner H, Oster H
J Endocrinol. 2021 Jun 28;250(2):67-79. doi: 10.1530/JOE-20-0611.
Toxicogenomic differentiation of functional responses to fipronil and imidacloprid in Daphnia magna
Pfaff J, Reinwald H, Ayobahan SU, Alvincz J, Göckener B, Shomroni O, Salinas G, Düring RA, Schäfers C, Eilebrecht S
Aquat Toxicol 2021 Jul 27;238:105927. doi: 10.1016/j.aquatox.2021.105927. Online ahead of print.
The Redox Homeostasis of Skeletal Muscle Cells Regulates Stage Differentiation of Toxoplasma gondii
Rahman MT, Swierzy IJ, Downie B, Salinas G, Blume M, McConville MJ, Lüder CGK
Front Cell Infect Microbiol 2021 Nov 22;11:798549. doi: 10.3389/fcimb.2021.798549. eCollection 2021.
Toxicogenomic fin(ger)prints for thyroid disruption AOP refinement and biomarker identification in zebrafish embryos
Reinwald H, König A, Ayobahan SU, Alvincz J, Sipos L, Göckener B, Böhle G, Shomroni O, Hollert H, Salinas G, Schäfers C, Eilebrecht E, Eilebrecht S
Sci Total Environ 2021 Mar 15;760:143914. doi: 10.1016/j.scitotenv.2020.143914. Epub 2020 Dec 10.
Biallelic variants in YRDC cause a developmental disorder with progeroid features
Schmidt J, Goergens J, Pochechueva T, Kotter A, Schwenzer N, Sitte M, Werner G, Altmüller J, Thiele H, Nürnberg P, Isensee J, Li Y, Müller C, Leube B, Reinhardt HC, Hucho T, Salinas G, Helm M, Jachimowicz RD, Wieczorek D, Kohl T, Lehnart SE, Yigit G, Wollnik B
Hum Genet 2021 Sep 20. doi: 10.1007/s00439-021-02347-3. Epub ahead of print.
Prognostic factor identification by screening changes in differentially expressed genes in oral squamous cell carcinoma
Schminke B, Shomroni O, Salinas G, Bremmer F, Kauffmann P, Schliephake H, Oyelami F, Rahat MA, Brockmeyer P
Oral Dis. 2021 Apr 19. doi: 10.1111/odi.13879. Epub ahead of print
Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
Sertel SM, Blumenstein W, Mandad S, Shomroni O, Salinas G, Rizzoli SO
Sci Rep 2021 Aug 30;11(1):17374. doi: 10.1038/s41598-021-96846-y.
The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models
Stegmann KM, Dickmanns A, Gerber S, Nikolova V, Klemke L, Manzini V, Schlösser D, Bierwirth C, Freund J, Sitte M, Lugert R, Salinas G, Meister TL, Pfaender S, Görlich D, Wollnik B, Groß U, Dobbelstein M
Virus Res 2021 Jun 3:198469. doi: 10.1016/j.virusres.2021.198469. Epub ahead of print.
CHARGE syndrome and related disorders: A mechanistic link
Ufartes R, Grün R, Salinas G, Sitte M, Kahl F, Wong MTY, van Ravenswaaij-Arts CMA, Pauli S
Hum Mol Genet 2021 Jul 6:ddab183. doi: 10.1093/hmg/ddab183. Epub ahead of print.
Post-transcriptional regulation by the exosome complex is required for cell survival and forebrain development via repression of P53 signaling
Ulmke PA, Xie Y, Sokpor G, Pham L, Shomroni O, Berulava T, Rosenbusch J, Basu U, Fischer A, Nguyen HP, Staiger JF, Tuoc T
Development. 2021 Feb 8;148(3):dev188276. doi: 10.1242/dev.188276.
Regenerative potential of epicardium-derived extracellular vesicles mediated by conserved miRNA transfer
Villa Del Campo C, Liaw NY, Gunadasa-Rohling M, Matthaei M, Braga L, Kennedy T, Salinas G, Voigt N, Giacca M, Zimmermann WH, Riley PR
Cardiovasc Res. 2021 Feb 18:cvab054. doi: 10.1093/cvr/cvab054. Epub ahead of print.
Transmitted resistance in HIV-1 of patients from nine departments of Peru
Yabar CA, Vilcarino GF, Espetia S, Yaya MG, Salinas G, García-Fernández L, Vásquez Becerra RD, Benites C, Santos-Anaya D, Mamani E, Acuña M, Romero S, Cárdenas F
Rev Peru Med Exp Salud Publica 2021 Jan-Mar;38(1):77-82. doi: 10.17843/rpmesp.2021.381.5527. Epub 2021 Jun 25.
EVL regulates VEGF receptor-2 internalization and signaling in developmental angiogenesis
Zink J, Frye M, Frömel T, Carlantoni C, John D, Schreier D, Weigert A, Laban H, Salinas G, Stingl H, Günther L, Popp R, Hu J, Vanhollebeke B, Schmidt H, Acker-Palmer A, Renné T, Fleming I, Benz PM
EMBO Rep 2021 Feb 3;22(2):e48961. doi: 10.15252/embr.201948961. Epub 2021 Jan 29.
2020
SUMOylation controls the neurodevelopmental function of the transcription factor Zbtb20
Ripamonti S, Shomroni O, Rhee JS, Chowdhury K, Jahn O, Hellmann KP, Bonn S, Brose N, Tirard M
J Neurochem 2020 Sep;154(6):647-661. doi: 10.1111/jnc.15008. Epub 2020 Apr 16.
Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction
Michel N, Narayanan P, Shomroni O, Schmidt M
Cell Rep 2020 Jul 21;32(3):107912. doi: 10.1016/j.celrep.2020.107912.
Long-term effects of empagliflozin on excitation-contraction-coupling in human induced pluripotent stem cell cardiomyocytes
Pabel S, Reetz F, Dybkova N, Shomroni O, Salinas G, Mustroph J, Hammer KP, Hasenfuss G, Hamdani N, Maier LS, Streckfuss-Bömeke K, Sossalla S
J Mol Med 2020 Oct 9. [Epub ahead of print]
Motor neuron translatome reveals deregulation of SYNGR4 and PLEKHB1 in mutant TDP-43 amyotrophic lateral sclerosis models
Marques RF, Engler JB, Küchler K, Jones RA, Lingner T, Salinas G, Gillingwater TH, Friese MA, Duncan KE
Hum Mol Genet 2020 Jul 7:ddaa140. doi: 10.1093/hmg/ddaa140. Online ahead of print.
Characterization of circulating breast cancer cells with tumorigenic and metastatic capacity
Koch C, Kuske A, Joosse SA, Yigit G, Sflomos G, Thaler S, Smit DJ, Werner S, Borgmann K, Gärtner S, Mossahebi Mohammadi P, Battista L, Cayrefourcq L, Altmüller J, Salinas-Riester G, Raithatha K, Zibat A, Goy Y, Ott L, Bartkowiak K, Tan TZ, Zhou Q, Speicher MR, Müller V, Gorges TM, Jücker M, Thiery JP, Brisken C, Riethdorf S, Alix-Panabières C, Pantel K.
EMBO Mol Med 2020 Jul 15:e11908. doi: 10.15252/emmm.201911908. Online ahead of print.
Intronic CRISPR Repair in a Preclinical Model of Noonan Syndrome-Associated Cardiomyopathy
Hanses U, Kleinsorge M, Roos L, Yigit G, Li Y, Barbarics B, El-Battrawy I, Lan H, Tiburcy M, Hindmarsh R, Lenz C, Salinas G, Diecke S, Müller C, Adham I, Altmüller J, Nürnberg P, Paul T, Zimmermann WH, Hasenfuss G, Wollnik B, Cyganek L.
Circulation. 2020 Jul 6. doi: 10.1161/CIRCULATIONAHA.119.044794. Online ahead of print.
DNA Methylation-Mediated Modulation of Endocytosis as Potential Mechanism for Synaptic Function Regulation in Murine Inhibitory Cortical Interneurons
Pensold D, Reichard J, Van Loo KMJ, Ciganok N, Hahn A, Bayer C, Liebmann L, Groß J, Tittelmeier J, Lingner T, Salinas-Riester G, Symmank J, Halfmann C, González-Bermúdez L, Urbach A, Gehrmann J, Costa I, Pieler T, Hübner CA, Vatter H, Kampa B, Becker AJ, Zimmer-Bensch G.
Cereb Cortex 2020 Jun 1;30(7):3921-3937. doi: 10.1093/cercor/bhaa009.
IgG Fc sialylation is regulated during the germinal center reaction upon immunization with different adjuvants
Bartsch YC, Eschweiler S, Leliavski A, Lunding HB, Wagt S, Petry J, Lilienthal GM, Rahmöller J, de Haan N, Hölscher A, Erapaneedi R, Giannou AD, Aly L, Sato R, de Neef LA, Winkler A, Braumann D, Hobusch J, Kuhnigk K, Krémer V, Steinhaus M, Blanchard V, Gemoll T, Habermann JK, Collin M, Salinas G, Manz RA, Fukuyama H, Korn T, Waisman A, Yogev N, Huber S, Rabe B, Rose-John S, Busch H, Berberich-Siebelt F, Hölscher C, Wuhrer M, Ehlers M.
J Allergy Clin Immunol 2020 May 20:S0091-6749(20)30728-4. doi: 10.1016/j.jaci.2020.04.059.
De novo mutations in FBRSL1 cause a novel recognizable malformation and intellectual disability syndrome
Ufartes R, Berger H, Till K, Salinas G, Sturm M, Altmüller J, Nürnberg P, Thiele H, Funke R, Apeshiotis N, Langen H, Wollnik B, Borchers A, Pauli S.
Hum Genet. 2020 May 18.
Inhibition of the autophagic protein ULK1 attenuates axonal degeneration in vitro and in vivo, enhances translation, and modulates splicing
Vahsen BF, Ribas VT, Sundermeyer J, Boecker A, Dambeck V, Lenz C, Shomroni O, Caldi Gomes L, Tatenhorst L, Barski E, Roser AE, Michel U, Urlaub H, Salinas G, Bähr M, Koch JC, Lingor P
Cell Death Differ 2020 Apr 27. doi: 10.1038/s41418-020-0543-y. [Epub ahead of print]
Diversity of Clinically Relevant Outcomes Resulting from Hypofractionated Radiation in Human Glioma Stem Cells Mirrors Distinct Patterns of Transcriptomic Changes
Kalasauskas D, Sorokin M, Sprang B, Elmasri A, Viehweg S, Salinas G, Opitz L, Rave-Fraenk M, Schulz-Schaeffer W, Kantelhardt SR, Giese A, Buzdin A, Kim EL
Cancers (Basel) 2020 Mar 1;12(3). pii: E570. doi: 10.3390/cancers12030570.
Ammonium acts systemically while nitrate exerts an additional local effect on Medicago truncatula nodules
Schulze J, Liese R, Ballesteros G, Casieri L, Salinas G, Cabeza RA
Plant Sci. 2020 Mar;292:110383. doi: 10.1016/j.plantsci.2019.110383. Epub 2019 Dec 19.
Increased presence and differential molecular imprinting of transit amplifying cells in psoriasis
Witte K, Jürchott K, Christou D, Hecht J, Salinas G, Krüger U, Klein O, Kokolakis G, Witte-Händel E, Mössner R, Volk HD, Wolk K, Sabat R
J Mol Med 2020 Jan;98(1):111-122. doi: 10.1007/s00109-019-01860-3. Epub 2019 Dec 12.
CRISPR-Mediated Activation of Endogenous Gene Expression in the Postnatal Heart
Schoger E, Carroll KJ, Iyer LM, McAnally JR, Tan W, Liu N, Noack C, Shomroni O, Salinas G, Groß J, Herzog N, Doroudgar S, Bassel-Duby R, Zimmermann WH, Zelarayán LC
Circ Res. 2020 Jan 3;126(1):6-24. doi: 10.1161/CIRCRESAHA.118.314522. Epub 2019 Nov 15.
2019
Sleep Loss Disrupts Morning-to-Evening Differences in Human White Adipose Tissue Transcriptome
Wilms B, Leineweber EM, Mölle M, Chamorro R, Pommerenke C, Salinas-Riester G, Sina C, Lehnert H, Oster H, Schmid SM.
J Clin Endocrinol Metab. 2019 May 1;104(5):1687-1696.
Nuclear Pre-snRNA Export Is an Essential Quality Assurance Mechanism for Functional Spliceosomes
Becker D, Hirsch AG, Bender L, Lingner T, Salinas G, Krebber H.
Cell Rep. 2019 Jun 11;27(11):3199-3214.e3.
Bassoon proteinopathy drives neurodegeneration in multiple sclerosis
Schattling B, Engler JB, Volkmann C, Rothammer N, Woo MS, Petersen M, Winkler I, Kaufmann M, Rosenkranz SC, Fejtova A, Thomas U, Bose A, Bauer S, Träger S, Miller KK, Brück W, Duncan KE, Salinas G, Soba P, Gundelfinger ED, Merkler D, Friese MA.
Nat Neurosci. 2019 Jun;22(6):887-896.
Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells
Page N, Klimek B, De Roo M, Steinbach K, Soldati H, Lemeille S, Wagner I, Kreutzfeldt M, Di Liberto G, Vincenti I, Lingner T, Salinas G, Brück W, Simons M, Murr R, Kaye J, Zehn D, Pinschewer DD, Merkler D.
Immunity. 2019 Mar 19;50(3):763.
The IL-1 Pathway Is Hyperactive in Hidradenitis Suppurativa and Contributes to Skin Infiltration and Destruction
Witte-Händel E, Wolk K, Tsaousi A, Irmer ML, Mößner R, Shomroni O, Lingner T, Witte K, Kunkel D, Salinas G, Jodl S, Schmidt N, Sterry W, Volk HD, Giamarellos-Bourboulis EJ, Pokrywka A, Döcke WD, Schneider-Burrus S, Sabat R.
J Invest Dermatol. 2019 Jun;139(6):1294-1305.
2018 and before
IL-12 and IL-15 induce the expression of CXCR6 and CD49a on peripheral natural killer cells
Hydes T, Noll A, Salinas-Riester G, Abuhilal M, Armstrong T, Hamady Z, Primrose J, Takhar A, Walter L, Khakoo SI.
Immun Inflamm Dis. 2018 Mar;6(1):34-46.
PI3K: A master regulator of brain metastasis-promoting macrophages/microglia
Blazquez R, Wlochowitz D, Wolff A, Seitz S, Wachter A, Perera-Bel J, Bleckmann A, Beißbarth T, Salinas G, Riemenschneider MJ, Proescholdt M, Evert M, Utpatel K, Siam L, Schatlo B, Balkenhol M, Stadelmann C, Schildhaus HU, Korf U, Reinz E, Wiemann S, Vollmer E, Schulz M, Ritter U, Hanisch UK, Pukrop T.
Glia. 2018 Nov;66(11):2438-2455.
Immortalization of common marmoset monkey fibroblasts by piggyBac transposition of hTERT
Petkov S, Kahland T, Shomroni O, Lingner T, Salinas G, Fuchs S, Debowski K, Behr R.
PLoS One. 2018 Sep 27;13(9):e0204580. doi: 10.1371/journal.pone.0204580. eCollection 2018.
Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping
Di Liberto G, Pantelyushin S, Kreutzfeldt M, Page N, Musardo S, Coras R, Steinbach K, Vincenti I, Klimek B, Lingner T, Salinas G, Lin-Marq N, Staszewski O, Costa Jordão MJ, Wagner I, Egervari K, Mack M, Bellone C, Blümcke I, Prinz M, Pinschewer DD, Merkler D.
Cell. 2018 Oct 4;175(2):458-471.e19.
Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes
Cyganek L, Tiburcy M, Sekeres K, Gerstenberg K, Bohnenberger H, Lenz C, Henze S, Stauske M, Salinas G, Zimmermann WH, Hasenfuss G, Guan K.
JCI Insight. 2018 Jun 21;3(12). pii: 99941.
Protein arginine methyltransferase 6 controls erythroid gene expression and differentiation of human CD34+ progenitor cells
Herkt SC, Kuvardina ON, Herglotz J, Schneider L, Meyer A, Pommerenke C, Salinas-Riester G, Seifried E, Bonig H, Lausen J.
Haematologica. 2018 Jan;103(1):18-29
Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells
Page N, Klimek B, De Roo M, Steinbach K, Soldati H, Lemeille S, Wagner I, Kreutzfeldt M, Di Liberto G, Vincenti I, Lingner T, Salinas G, Brück W, Simons M, Murr R, Kaye J, Zehn D, Pinschewer DD, Merkler D.
Immunity. 2018 May 15;48(5):937-950.e8.
Epithelial-Mesenchymal Transition during Metastasis of HPV-Negative Pharyngeal Squamous Cell Carcinoma
Ihler F, Gratz R, Wolff HA, Weiss BG, Bertlich M, Kitz J, Salinas G, Rave-Fränk M, Canis M.
Biomed Res Int. 2018 Mar 6;2018:7929104.
Effects of repeated long-term psychosocial stress and acute cannabinoid exposure on mouse corticostriatal circuitries: Implications for neuropsychiatric disorders
Tomas-Roig J, Piscitelli F, Gil V, Quintana E, Ramió-Torrentà LL, Del Río JA, Moore TP, Agbemenyah H, Salinas G, Pommerenke C, Lorenzen S, Beißbarth T, Hoyer-Fender S, Di Marzo V, Havemann-Reinecke U.
CNS Neurosci Ther. 2018 Jun;24(6):528-538.
The DNA Methyltransferase 1 (DNMT1) Controls the Shape and Dynamics of Migrating POA-Derived Interneurons Fated for the Murine Cerebral Cortex.
Pensold D, Symmank J, Hahn A, Lingner T, Salinas-Riester G, Downie BR, Ludewig F, Rotzsch A, Haag N, Andreas N, Schubert K, Hübner CA, Pieler T, Zimmer G.
Cereb Cortex. 2017 Dec 1;27(12):5696-5714.
Hepatic gene therapy rescues high-fat diet responses in circadian Clock mutant mice
Meyer-Kovac J, Kolbe I, Ehrhardt L, Leliavski A, Husse J, Salinas G, Lingner T, Tsang AH, Barclay JL, Oster H.
Mol Metab. 2017 Mar 29;6(6):512-523.
The adaptation of colorectal cancer cells when forming metastases in the liver: expression of associated genes and pathways in a mouse model
BBocuk D, Wolff A, Krause P, Salinas G, Bleckmann A, Hackl C, Beissbarth T, Koenig S.
BMC Cancer. 2017 May 19;17(1):342.
The β-catenin/CBP-antagonist ICG-001 inhibits pediatric glioma tumorigenicity in a Wnt-independent manner
Wiese M, Walther N, Diederichs C, Schill F, Monecke S, Salinas G, Sturm D, Pfister SM, Dressel R, Johnsen SA, Kramm CM.
Oncotarget. 2017 Apr 18;8(16):27300-27313.
Histone deacetylase inhibitor MS-275 augments expression of a subset of IFN-γ-regulated genes in Toxoplasma gondii-infected macrophages but does not improve parasite control
Sumpf K, Nast R, Downie B, Salinas G, Lüder CG.
Exp Parasitol. 2017 Feb 9. pii: S0014-4894(17)30083-8.
mRNA quality control is bypassed for immediate export of stress-responsive transcripts
Zander G, Hackmann A, Bender L, Becker D, Lingner T, Salinas G, Krebber H.
Nature. 2016 Dec 12.
The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code
Hofhuis J, Schueren F, Nötzel C, Lingner T, Gärtner J, Jahn O, Thoms S.
Open Biol. 2016 Nov;6(11).
Pre-Infection Transcript Levels of FAM26F in PBMCS Inform about Overall Plasma Viral Load in Acute and Postacute Phase after SIV-Infection
Javed A, Leuchte N, Salinas G, Opitz L, Stahl-Hennig C, Sopper S, Sauermann U.
J Gen Virol. 2016 Oct 18.
Identification of New Fungal Peroxisomal Matrix Proteins and Revision of the PTS1 Consensus
Nötzel C, Lingner T, Klingenberg H, Thoms S.
Traffic. 2016 Oct;17(10):1110-24
Arc/Arg3.1 governs inflammatory dendritic cell migration from the skin and thereby controls T cell activation
Ufer F, Vargas P, Engler JB, Tintelnot J, Schattling B, Winkler H, Bauer S, Kursawe N, Willing A, Keminer O, Ohana O, Salinas-Riester G, Pless O, Kuhl D, Friese MA.
Sci Immunol. 2016 Sep 23;1(3):eaaf8665.
Treatment of prostate cancer cells with S-adenosylmethionine leads to genome-wide alterations in transcription profiles
Schmidt T, Leha A, Salinas-Riester G.
Gene. 2016 Sep 26. pii: S0378 1119(16)30770-3
The SCN Clock Governs Circadian Transcription Rhythms in Murine Epididymal White Adipose Tissue
Kolbe I, Husse J, Salinas G, Lingner T, Astiz M, Oster H.
J Biol Rhythms. 2016 Sep 20. pii: 0748730416666170
Effects of Long-Term Environmental Enrichment on Anxiety, Memory, Hippocampal Plasticity and Overall Brain Gene Expression in C57BL6 Mice
Hüttenrauch M, Salinas G, Wirths O.
Front Mol Neurosci. 2016 Aug 3;9:62
Prognostic Value of MicroRNAs in Preoperative Treated Rectal Cancer
Azizian A, Epping I, Kramer F, Jo P, Bernhardt M, Kitz J, Salinas G, Wolff HA, Grade M, Beißbarth T, Ghadimi BM, Gaedcke J.
Int J Mol Sci. 2016 Apr 15;17(4):568
The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features
Debowski K, Drummer C, Lentes J, Cors M, Dressel R, Lingner T, Salinas-Riester G, Fuchs S, Sasaki E, Behr R.
Sci Rep. 2016 Jul 7;6:29122
Physical activity delays hippocampal neurodegeneration and rescues memory deficits in an Alzheimer disease mouse model
Hüttenrauch M, Brauß A, Kurdakova A, Borgers H, Klinker F, Liebetanz D, Salinas-Riester G, Wiltfang J, Klafki HW, Wirths O.
Transl Psychiatry. 2016 May 3;6:e800
MiR144/451 Expression Is Repressed by RUNX1 During Megakaryopoiesis and Disturbed by RUNX1/ETO
DKohrs N, Kolodziej S, Kuvardina ON, Herglotz J, Yillah J, Herkt S, Piechatzek A, Salinas Riester G, Lingner T, Wichmann C, Bonig H, Seifried E, Platzbecker U, Medyouf H, Grez M, Lausen J.
PLoS Genet. 2016 Mar 18;12(3):e1005946
The Cannabinoid CB1/CB2 Agonist WIN55212.2 Promotes Oligodendrocyte Differentiation In Vitro and Neuroprotection During the Cuprizone-Induced Central Nervous System Demyelination
Tomas-Roig J, Wirths O, Salinas-Riester G, Havemann-Reinecke U.
CNS Neurosci Ther. 2016 May; 22(5):387-95
Social defeat leads to changes in the endocannabinoid system: An overexpression of calreticulin and motor impairment in mice
Tomas-Roig J, Piscitelli F, Gil V, Del Río JA, Moore TP, Agbemenyah H, Salinas-Riester G, Pommerenke C, Lorenzen S, Beißbarth T, Hoyer-Fender S, Di Marzo V, Havemann-Reinecke U.
Behav Brain Res. 2016 Apr 15;303:34-43
Short-Term Molecular Acclimation Processes of Legume Nodules to Increased External Oxygen Concentration
Avenhaus U, Cabeza RA, Liese R, Lingner A, Dittert K, Salinas-Riester G, Pommerenke C, Schulze J.
Front Plant Sci. 2016 Jan 6;6:1133. doi: 10.3389/fpls.2015.01133. eCollection 2015
CD14 is a key organizer of microglial responses to CNS infection and injury
Janova H, Böttcher C, Holtman IR, Regen T, van Rossum D, Götz A, Ernst AS, Fritsche C, Gertig U, Saiepour N, Gronke K, Wrzos C, Ribes S, Rolfes S, Weinstein J, Ehrenreich H, Pukrop T, Kopatz J, Stadelmann C, Salinas-Riester G, Weber MS, Prinz M, Brück W, Eggen BJ, Boddeke HW, Priller J, Hanisch UK.
Glia. 2016 Apr;64(4):635-49
Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary
Fereydouni B, Salinas-Riester G, Heistermann M, Dressel R, Lewerich L, Drummer C, Behr R.
Stem Cells Int. 2016;2016:2480298
Modulation of CNS autoimmune responses by CD8(+) T cells coincides with their oligoclonal expansion
Fischer HJ, van den Brandt J, Lingner T, Odoardi F, Flügel A, Weishaupt A, Reichardt HM.
J Neuroimmunol. 2016 Jan 15;290:26-32
Microarray analysis of circulating micro RNAs in the serum of patients with polymyositis and dermatomyositis reveals a distinct disease expression profile and is associated with disease activity
Misunova M, Salinas-Riester G, Luthin S, Pommerenke C, Husakova M, Zavada J, Klein M, Plestilova L, Svitalkova T, Cepek P, Novota P, Vencovsky J.
Clin Exp Rheumatol. 2016 Jan-Feb;34(1):17-24. Epub 2015 Nov 17
SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
Zautner AE, Goldschmidt AM, Thürmer A, Schuldes J, Bader O, Lugert R, Groß U, Stingl K, Salinas G, Lingner T.
BMC Genomics. 2015 Dec 21;16:1088
Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species
Claußen M, Lingner T, Pommerenke C, Opitz L, Salinas G, Pieler T.
Mol Biol Cell. 2015 Nov 1;26(21):3777-87
Thymocyte-derived BDNF influences T-cell maturation at the DN3/DN4 transition stage
Linker RA, Lee DH, Flach AC, Litke T, van den Brandt J, Reichardt HM, Lingner T, Bommhardt U, Sendtner M, Gold R, Flügel A, Lühder F.
Eur J Immunol. 2015 May;45(5):1326-38
Central role of Th2/Tc2 lymphocytes in pattern II multiple sclerosis lesions
Planas R, Metz I, Ortiz Y, Vilarrasa N, Jelčić I, Salinas-Riester G, Heesen C, Brück W, Martin R, Sospedra M.
Ann Clin Transl Neurol. 2015 Sep;2(9):875-93
Influence of total genomic alteration and chromosomal fragmentation on response to a combination of azacitidine and lenalidomide in a cohort of patients with very high risk MDS
Ganster C, Shirneshan K, Salinas-Riester G, Braulke F, Schanz J, Platzbecker U, Haase D.
Leuk Res. 2015 Oct;39(10):1079-87
Sensing Cardiac Electrical Activity With a Cardiac Myocyte-Targeted Optogenetic Voltage Indicator
Chang Liao ML, de Boer TP, Mutoh H, Raad N, Richter C, Wagner E, Downie BR, Unsöld B, Arooj I, Streckfuss-Bömeke K, Döker S, Luther S, Guan K, Wagner S, Lehnart SE, Maier LS, Stühmer W, Wettwer E, van Veen T, Morlock MM, Knöpfel T, Zimmermann WH.
Circ Res. 2015 Aug 14;117(5):401-12
Next generation sequencing of sex-specific genes in the livers of obese ZSF1 rats
Babelova A, Burckhardt BC, Salinas-Riester G, Pommerenke C, Burckhardt G, Henjakovic M.
Genomics. 2015 Jul 19. pii: S0888-7543(15) 30021-5
The oxidative demethylase ALKBH3 marks hyperactive gene promoters in human cancer cells
Liefke R, Windhof-Jaidhauser IM, Gaedcke J, Salinas-Riester G, Wu F, Ghadimi M, Dango S.
Genome Med. 2015 Jun 30;7(1):66
Thymocyte-derived BDNF influences T-cell maturation at the DN3/DN4 transition stage
Linker RA, Lee DH, Flach AC, Litke T, van den Brandt J, Reichardt HM, Lingner T, Bommhardt U, Sendtner M, Gold R, Flügel A, Lühder F.
Eur J Immunol. 2015 May;45(5):1326-38
Destabilization of pluripotency in the absence of Mad2l2
Pirouz M, Rahjouei A, Shamsi F, Eckermann KN, Salinas-Riester G, Pommerenke C, Kessel M.
Cell Cycle. 2015 Apr 30:0
Overexpression of mutant Ptch in rhabdomyosarcomas is associated with promoter hypomethylation and increased Gli1 and H3K4me3 occupancy
Nitzki F, Tolosa EJ, Cuvelier N, Frommhold A, Salinas-Riester G, Johnsen SA, Fernandez-Zapico ME, Hahn H.
Oncotarget. 2015 Apr 20;6(11):9113-24
Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach
Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R, Langenstroth D, Gromoll J, Sasaki E, Behr R.
PLoS One. 2015 Mar 18;10(3):e0118424
mRNA profiling reveals determinants of trastuzumab efficiency in HER2-positive breast cancer
von der Heyde S, Wagner S, Czerny A, Nietert M, Ludewig F, Salinas-Riester G, Arlt D, Beißbarth T.
PLoS One. 2015 Feb 24;10(2):e0117818
Integrated miRNA and mRNA profiling of tumor-educated macrophages identifies prognostic subgroups in estrogen receptor-positive breast cancer
Bleckmann A, Leha A, Artman S, Menk K, Salinas-Riester G, Binder C, Pukrop T, Beissbarth T, Klemm F.
Mol Oncol. 2014 Aug 16. pii: S1574-7891(14)00178-1